Figure 2.

Effect of APEX2 construct expression on inclusion diameter. HeLa cells were infected with Ctr L2 transformants (detailed on the x-axis) as in Figure 1, and construct expression was induced with the indicated concentrations of anhydrotetracycline (aTc) at 7 h post-infection. Cells were fixed 36 h post-infection, processed for indirect immunofluorescence, and inclusion diameters (expressed in μm, y-axis) were determined as in Materials and Methods. Data shown include two independent experiments where 100 inclusions/coverslip were evaluated. Inclusion diameter mean and standard error of the mean are shown and were graphed using GraphPad Prism 7.0. Statistical analysis of the data included a one-way ANOVA with Tukey's multiple comparisons test; statistical significance is shown with ^**** equaling p < 0.0001, and # equaling p < 0.0001 for all conditions specifically compared to IncF-APEX2 induced with 5 nM aTc.