Fig. 3.

Adhesion of MSC95-FimH to cells under static conditions. a FACS analysis demonstrates that GFP-labelled FimH-positive E. coli (MSC95-FimH) adhere to the normal bladder epithelial cell line SV-HUC under static conditions (appearance of GFP positive peak, left panel), while FimH-negative E. coli (MSC95) (absence of GFP positive peak, middle panel) do not. Right panel SV-HUC alone. b MSC95-FimH (white boxes, FimH+) adhere better to human dermal microvascular (G1S1) and immortalised glomerular endothelial cells (GEnC) as analysed by FACS than to SV-HUC, and only poorly to malignant urothelial cells (5637 and HT-1376). MSC95 (FimH-negative) confirms that adherence is FimH-dependent. *p < 0.05, **p < 0.01, Kruskal–Wallis test followed by two-tailed Mann–Whitney test. Adhesion was determined to each cell line in at least four experiments in triplicate