Table 1.
Strains and plasmids used in this study
| Strain or plasmid | Description, genotype, or sequence | Reference, source |
|---|---|---|
| Strains | ||
| E. coli K-12 MG1655 | F− l− ilvG rfb-50 rph-1 | CGSC Collection |
| E. coli WC196LC | W3110 NTG mutant (S-aminoethyl-L-cysteine-resistant mutant) Δldc ΔcadA | Kikuchi et al. (1997) |
| Bacillus subtilis 168 | trpC2 ypqP::SPβ | Zeigler et al. (2008) |
| MG1655 (empty vector) | E. coli K-12 MG1655 harboring pTWV229 and pMW219-Δplac | This study |
| MG1655 (glvAC) | E. coli K-12 MG1655 harboring pTWV229-self-glvA-Fw and pMW219-ΔPlac-*tac-glvC | This study |
| WC196LC (pCABD2) | E. coli WC196LC harboring pCABD2 | Kojima et al. (1994); Kikuchi et al. (1997); Doi et al. (2014) |
| Plasmids | ||
| pTWV229 | Cloning vector, Apr | Takara Bio Inc., (Japan) |
| pMW219 | Cloning vector, Kmr | Nippon Gene Co. Ltd. (Japan) |
| pMW219-ΔPlac | pMW219 derivative lacking the lac promoter region | This study |
| pMW219-ΔPlac-tac-glvC-R2 | pMW219-ΔPlac derivative harboring the Ptac-glvC gene | This study |
| pMW219-ΔPlac-Ptac4075-glvC-Rv | pMW219-ΔPlac derivative harboring the Ptac4075-glvC gene | This study |
| pTWV229-self-glvA-Fw | pTWV229 derivative harboring the Pself-glvA gene | This study |
| pMW219-ΔPlac-*tac-glvC | pMW219-ΔPlac-tac-glvC-R2 derivative harboring a mutation in the −10 region of the tac promoter (TATAAT to AATAAT) | This study |
| pCABD2 | pRSF1010 harboring mutated lysC, mutated dapA, mutated dapB, and C. glutamicum ddh | Kojima et al. (1994) |