Table 3.
Growth of E. coli recombinants on selected sugars
| Substrate | DP | Form | MG1655 (empty vector) | MG1655 (glvAC) | MG1655 (pAP2) (Pikis et al. 2006) |
|---|---|---|---|---|---|
| Glucose | 1 | Sugar | ++ | ++ | ++ |
| α-Methyl-glucoside | 1 | Sugar | NDG | ++ | ++ |
| Sucrose | 2 | Sugar | NDG | ++ | − |
| Maltose | 2 | Sugar | ++ | ++ | ++ |
| Isomaltose | 2 | Sugar | NDG | ++ | ++ |
| Maltitol | 2 | Sugar alcohol | NDG | ++ | ++ |
| Trehalose | 2 | Sugar | ++ | ++ | ++ |
| Turanose | 2 | Sugar | + | ++ | ++ |
| Maltulose | 2 | Sugar | NDG | ++ | ++ |
| Isomaltulose | 2 | Sugar | NDG | ++ | ++ |
| Galactinol | 2 | Sugar alcohol | NDG | NDG | No information |
| Cellobiose | 2 | Sugar | + | + | No information |
| Gentiobiose | 2 | Sugar | NDG | + | No information |
| Lactose | 2 | Sugar | ++ | ++ | No information |
| Melibiose | 2 | Sugar | ++ | ++ | No information |
| Lactulose | 2 | Sugar | ++ | ++ | No information |
| Maltotriose | 3 | Sugar | ++ | ++ | No information |
| Panose | 3 | Sugar | NDG | ++ | No information |
| Isomaltotriose | 3 | Sugar | NDG | ++ | No information |
| Maltotetraose | 4 | Sugar | ++ | ++ | No information |
| Raffinose | 3 | Sugar | + | ++ | No information |
| Maltotriitol | 3 | Sugar alcohol | NDG | NDG | No information |
| Isomaltitol | 3 | Sugar alcohol | NDG | ++ | No information |
| Lactitol | 2 | Sugar alcohol | ++ | ++ | No information |
| Erlose | 3 | Sugar | NDG | NDG | No information |
| Maltopentaose | 5 | Sugar | ++ | ++ | No information |
Data of MG1655 (pAP2) which expresses aglA and aglB from K. pneumoniae are described in the reference (Pikis et al. 2006) and listed in this table for comparison to show what kinds of sugars and sugar alcohols were newly assimilated by heterologous expression of glvA and glvC
DP degree of polymerization, NDG no detectable growth, − minimal growth, + slight growth, ++ clear growth