Fig. 5.

P54nrb and PSPC1 modulate HSV-1 replication and gene expression. a HeLa cells transfected with P54nrb, PSPC1 or the control siRNA were infected with HSV-1 for 4 h. The samples were collected for western blot to analyze the expression of P54nrb, PSPC1 and β-actin. b The samples in a. were fixed and immuno-stained with an anti-HSV-1 glycoprotein antibody (green). Images were captured with a confocal microscope. Nuclei were stained with DAPI (blue). Scale bars 80 μm. c Plaque-forming assay in HeLa cells transfected with P54nrb, PSPC1 or the control siRNA and infected with HSV-1 for the indicated time points. d Western blot analysis of ICP0, TK and β-actin in HeLa cells transfected with P54nrb or PSPC1 siRNA and infected with HSV-1. e HeLa cells transfected with the indicated siRNAs were infected with HSV-1 for 4 h. The relative expression of ICP0 and TK relative to that of ACTB was measured with real-time PCR. f MEF cells transfected with the indicated siRNAs were infected with HSV-1 for 4 h. Samples were collected for western blot to analyze the expression of P54nrb, PSPC1, ICP0, and TK. g The samples in f were collected to analyze the mRNA levels of ICP0 and TK relative to that of ACTB. *p < 0.01