TABLE 2 .

Constraints applied to the metabolic network of P. tricornutum^a

Reaction ID	Applied constraint (mM)
Ex_hco3_e	LBb and UB,c −1.57 for low HCO3, −3.14 for high HCO3
Ex_no3_e	LB and UB, −0.535
Ex_biotin_e	LB, −1,000; UB, 0
Ex_fe2_e	LB, −1,000; UB, 0
Ex_h_e	LB, −1,000; UB, 1,000
Ex_h2o_e	LB, −1,000; UB, 1,000
Ex_o2_e	LB, −1,000; UB, 1,000
Ex_pi_e	LB, −0.22; UB, 0
Ex_na1_e	LB, −1,000; UB, 1,000
Ex_so4_e	LB, −28.8; UB, 0
Ex_mg2_e	LB, −1,000; UB, 0
Ex_cl_e	LB, −1,000; UB, 1,000
Ex_thm_e	LB, −1,000; UB, 0

^aConstraints were applied to nutrient uptake and product secretion for sampling and simulations as used in reference 6, except for HCO₃ instead of CO₂ uptake. Exchange reactions not shown here are blocked.

^bLB, lower bound.

^cUB, upper bound.