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. 2016 Dec 7;595(4):1039–1058. doi: 10.1113/JP273302

Figure 3. Store‐operated phosphorylation of TRPC1 proteins requires STIM1.

Figure 3

A, co‐immunoprecipitation of rabbit portal vein tissue lysates with anti‐phosphorylated serine (pSer) and threonine (pThr) antibodies followed by western blotting (WB) with an anti‐TRPC1 antibody shows that basal TRPC1 phosphorylation is increased by pre‐treatment with BAPTA‐AM for 10 min, and that this increase was reduced by STIM1 shRNA1 and coapplication of GF109203X. B, mean relative band densities normalized to BAPTA‐AM bands (three different tissue lysate preparations; * P < 0.05, ** P < 0.01). [Colour figure can be viewed at wileyonlinelibrary.com]