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. 2016 Dec 7;595(4):1039–1058. doi: 10.1113/JP273302

Figure 11. Noradrenaline‐evoked TRPC1 channel activity is mediated by STIM1.

Figure 11

A, bath application of noradrenaline evoked TRPC1 channel activity in a concentration‐dependent manner in cell‐attached patches from WT mesenteric artery VSMCs held at −80 mV, which were reduced in VSMCs expressing STIM1 shRNA1 and shRNA2 sequences compared to scrambled shRNA. B, mean data showing the inhibitory actions of STIM1 shRNA1 and shRNA2 on noradrenaline‐evoked TRPC1 channel activity (at least six patches in which every concentration of noradrenaline tested was cumulatively applied; ** P < 0.01, *** P < 0.001). C, PLA images from single WT VSMCs showing that application of noradrenaline for 5 min induced fluorescence signals (red), which indicated interactions between TRPC1 and STIM1, STIM1 and Gαq, and STIM1 and PLCβ1 predominantly at the PM.