Figure 3. Local application of physostigmine (PHYSO, 5 mm, 200 nl) in pFRG does not affect spontaneous brain state alternations.

A, long trace recordings of ABD_EMG, ∫ABD_EMG, respiratory airflow, hippocampal activity (HPC) and its power spectrogram during local bilateral application of PHYSO (grey lines indicate time of injections on the two sides of the ventrolateral medulla) during activated state (high power at 4 Hz frequency). Note lack of effect in spontaneous brain state alternation and the persistence of expiratory activity with brain state changes. B, long trace recordings of ABD_EMG, ∫ABD_EMG, respiratory airflow, cortical activity (CTX) and its power spectrogram during local bilateral application of PHYSO during deactivated state (high power at 1 Hz frequency). Note lack of effect in spontaneous brain state alternation and the onset of expiratory activity occurring with activated states (i.e., low power in the CTX 1 Hz frequency range).