Table 1.
Primers for pyrosequencing of Nos3 promoter
| Sequence | ||
|---|---|---|
| * CpGs –220 to –170 | Forward | 5’‐GTTTTTTATATAATGGGATAGGAATAAGGT‐3’ |
| Reverse | biotin5’‐CCCAACCAACCTTATTCCTAT‐3’ | |
| Sequencing | 5’‐GTTGGGAGGTTTTGAA‐3’ | |
| * CpGs –111 to –93 | Forward | 5’‐AAGTAGGGAGGGGGTTGAG‐3’ |
| Reverse | biotin5’‐CCCAACCAACCTTATTCCTAT‐3’ | |
| Sequencing | 5’‐GAGGGGAGGGGTATT‐3’ | |
| CpGs –27 to –11 | Forward | 5’‐AAGGAAAAGGTTAGGGTTTTGT‐3’ |
| Reverse | biotin5’‐TCCTAACCCACACTCTTAAAATTAC‐3’ | |
| Sequencing | 5’‐GTTAGGGTTTTGTTGGA‐3’ | |
| CpGs +34 to +46 | Forward | 5’‐AGAGTTGAAGGGAGGTTGATATGG‐3’ |
| Reverse | biotin5’‐AACCCTACTTACCACACAATCC‐3’ | |
| Sequencing | 5’‐TTAAGAGTGTGGGTTAG‐3’ |
*
This set of primers was designed on the complementary strand.