FIGURE 2.

Results of mycobiota analysis in the mock community using IonPGM sequencing. (A) Relative abundance of major fungal genera in the mock community shown in a bar graph. Read-trimming was carried out using an ideal relative abundance of the mock community and MQV 10 – 30. Percentage read abundances of 15 genera are shown in Supplementary Figure S4. (B) Relative abundances of the major fungal genera are shown in different colors according to the length of the ITS1 region. (C) MQV at each base position of the read. (D) Genus-level hierarchical clustering analysis of the relative abundance of the major fungal genera. Results of analyses conducted using MQV ranging from 10 to 30 were analyzed along with ideal values.