Table 2.
Primers used for real-time PCR.
| Name | Sequence (5′–3′) | Annealing temp (°C) |
|---|---|---|
| BECN1 | Sense: GGATGGTGTCTCTCGCAGAT | 59.8 |
| Antisense: CAGTCTTCGGCTGAGGTTCT | 59.8 | |
| LC3 | Sense: ACCCTGAGTCTTCTCTTCAGGT | 60.2 |
| Antisense: GTTGCGCTTCACAACTCAGG | 60.0 | |
| Atg5 | Sense: AGGCAACCTGACCAGAAACA | 57.8 |
| Antisense: GAGGAAAGCAGAGGTGATGC | 59.8 | |
| GADPH | Sense: CAGGAGGCATTGCTGATGAT | 58.4 |
| Antisense: GAAGGCTGGGGCTCATTT | 56.3 |
BECN1, beclin1 autophagy-related gene; LC3, microtubule-associated protein-light chain 3; Atg5, autophagy-related gene 5; GADPH, glyceraldehyde-3-phosphate dehydrogenase.