Figure 3.

Illustrates gene and protein expression of C2C12 murine myocyte incubated under 37°C (control) or 40^°C (heat stressed) for 24 h. (A) Metabolic gene expression (n = 3) of peroxisome proliferator‐activated receptor γ coactivator 1 alpha (PGC‐lα), nuclear respiratory factor 1 (NRF1), and mitochondrial transcription factor A (TFAM) were normalized to the housekeeping gene, TATA‐binding protein (TBP) and, (B) Protein expression (n = 3) of cells treated as described above of PGC‐lα. Significance was indicated as *, **, and *** indicating P < 0.05, P < 0.01, and P < 0.001 statistical differences compared to control, respectively. Data were standardized to the control and reported as percentage.