Figure 1.

ASIC1a‐mediated whole‐cell currents elicited by pH 5.5 were significantly increased in the presence of t‐DCA but were unaffected by t‐DCA pretreatment. (A, C, E), Representative whole‐cell current traces recorded in human ASIC1a expressing oocytes. Bath pH was 7.4 except for time intervals indicated by open bars during which bath pH was switched to 5.5. t‐DCA (500 μmol/L) was present in the bath solution as indicated by black bars. In the control experiment shown in C the 3rd and 4th pH 5.5 responses were elicited using a solution from a different reservoir to confirm the reproducibility of the responses. (B, D, F), Summary of results from similar experiments as shown in A (n = 34; N = 6), C (n = 15; N = 3), and E (n = 25; N = 2), respectively. Lines connect data points obtained in the same experiment. To quantify current responses, the integral of the inward current elicited by pH 5.5 below baseline was determined for each response. For the summary data shown in B and D mean current integral values of the 3rd and 4th response and of the 5th and 6th response were normalized to the mean current integral of the first two responses (Normalized current integral). For the summary data shown in F the current integral value of the 2nd response (after t‐DCA) was normalized to the current integral of the 1st response (before t‐DCA). *** p < 0.001; n.s. not significant; one‐way repeated measures ANOVA with Bonferroni post hoc test was used in B and D; Student's t‐test was used in F.