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. 2017 Feb 7;8:13876. doi: 10.1038/ncomms13876

Figure 2. Identifying HCIPs that regulate IAV infection by screening the core interactome.

Figure 2

(a) Map of the core IAV–host protein interactome that comprises HCIPs associated with multiple IAV strains in Fig. 1b. Legends are indicated. (b) PR8-Gluc reporter screening assay in HEK293 cells. At 24 h after transfection with HCIPs, the cells were infected with 0.1 MOI of PR8-Gluc. After 16 h, IAV infection activity was determined by luciferase activity. The screenings were biologically repeated two times. The dash lines indicate ±2 X s.d. of the whole screening data set. The HCIPs with >2 × s.d. or <−2 × s.d. are labelled. (c) A549 and primary human tracheal epithelial cells were transfected with control siRNA or the indicated siRNA duplexes. After 48 h, the cells were infected with 0.1 MOI of PR8-Gluc for 16 h. The relative luciferase activity was examined. Data represent means ±s.d. of three independent experiments. The P value was calculated (two-tailed Student's t-test) by comparison with the corresponding siRNA control in each cell group. An asterisk indicates P<0.05.