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. 2017 Feb 7;8:13876. doi: 10.1038/ncomms13876

Figure 3. PKP2 interacts and co-localizes with PB1.

Figure 3

(a) Primary human tracheal epithelial cells were infected with 1 MOI of PR8 IAV for 16 h. Cell lysates were subjected to immunoprecipitation and immunoblotting with the indicated antibodies to detect endogenous interactions. Molecular weights (MW) are indicated. (b) A549 cells were infected with 1 MOI of PR8 IAV for 8 h and stained with anti-PKP2 (green), anti-PB1 (red) and DAPI nuclear stain (blue). (c) Full-length and various PB1 deletion mutants were fused with FLAG epitope and co-transfected with PKP2-V5 into HEK293 cells. Lysates were immunoprecipitated with anti-FLAG antibody and blotted with the indicated antibodies. (d) Full-length and various PKP2 deletion mutants were fused with FLAG epitope and co-transfected with PB1-HA into HEK293 cells. Lysates were immunoprecipitated with anti-FLAG antibody and blotted with the indicated antibodies.