Table 3. Comparison of Illumina sequencing-based STR genotyping methods.
| Method | Array capture | MIPSTR | STR-Seq with random shearing | STR-Seq with CRISPR–Cas9 |
|---|---|---|---|---|
| Number of sample analysed | 8 | 96 | 3* | 5* |
| Target STR | 7,851 | 100 | 2,543 | 2,543 |
| Total successfully genotyped STR (% of all target) | 33,947 (54.0%) | 6,144 (64.0%) | 6,206 (81.3%) | 10,576 (83.2%) |
| Total sequencing reads, millions | 201.8 | 4.4 | 4.5 | 6.8 |
| STR genotype per million total reads | 168 | 1,390 | 1,376 | 1,545 |
| On-target reads, millions (% of total reads) | 77.3 (38.3%) | 4.0 (90.2%) | 3.7 (81.8%) | 5.5 (80.1%) |
| Informative reads, millions (% of on-target reads) | 4.4 (5.7%) | 3.0 (75.6%) | 0.4 (9.8%) | 1.0 (18.6%) |
| Reference | Guilmatre et al.26 | Carlson et al.31 | This study | This study |
*All the non-mixture samples analysed by the high stringency wash protocol.