Table 2. Kinetic parameters of wild-type and mutant proteins of Syn, human and rat BVRs* †.
| Variant | kcat (s−1) | Km (μM) | kcat/Km (s−1 μM−1) |
| Synechocystis sp. PCC 6803 | |||
| Wild-type | 7.1±0.2 | 1.8±0.3 | 3.9 |
| Y102F | 2.7±0.07 | 4.0±0.3 | 0.7 |
| T169A | 13.1±0.5 | 3.4±0.5 | 3.9 |
| S184A | 11.3±0.5 | 1.9±0.4 | 5.9 |
| R185A | 0.7±0.02 | 6.9±0.6 | 0.1 |
| R185K | 0.9±0.05 | 12.6±1.5 | 0.07 |
| R188A | 13.4±0.4 | 3.3±0.4 | 4.1 |
| K237A | 23.7±2.1 | 4.8±1.3 | 4.9 |
| R246A | 11.0±0.6 | 5.1±0.8 | 2.2 |
| Human | |||
| Wild-type | 94.8±31.4 | 20.7±10.1 | 4.6 |
| Y98F | 16.8±0.5 | 3.8±0.3 | 4.4 |
| R172A | 17.9±6.3 | 87.7±35.7 | 0.2 |
| R172E | N.D. | N.D. | — |
| R172K | N.D. | N.D. | — |
| Rat | |||
| Wild-type | 69.3±8.5 | 4.6±1.1 | 15.1 |
| Y97F | 13.6±0.6 | 11.7±1.0 | 1.2 |
| R171A | 6.0±0.2 | 24.4±1.5 | 0.2 |
| R171E | 0.7±0.2 | 45.7±15.4 | 0.02 |
| R171K | 14.7±8.5 | 163.3±103.2 | 0.1 |
*Errors (s.d.) are calculated from triplicate measurements.
†N.D., not detectable.