Figure 2.

Effect of Rb2 on GPR120 expression in RAW264.7 macrophages. RAW264.7 cells were incubated with Rb2 (0.1–100 μmol/L) or vehicle (DMSO) for 24 h. The expression levels of GPR120 protein (A) in cell lysates were detected by Western blot analysis and mRNA (B) in cells were detected by quantitative RT-PCR. The expression levels of GPR120 protein (C) and mRNA (D) in cells transfected with GPR120 shRNA or control shRNA were detected by Western blot analysis and quantitative RT-PCR, respectively. (E) The induction of GPR120 by Rb2 behaved in a time-dependent manner as determined by Western blot analysis. The data are presented as mean±SD of three independent experiments. ^*P<0.05 vs vehicle (DMSO) group, control shRNA or '0 h' group which acts as control.