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. 2016 Nov 21;38(2):264–276. doi: 10.1038/aps.2016.99

Figure 4.

Figure 4

Effects of SMI on the cytotoxicity of ADR or PTX in colon cancer cells. Caco-2 and LoVo colon cancers were treated with ADR or PTX with or without SMI for 48 h. (A) Effects of SMI on the cytotoxicity of ADR to Caco-2 cells. (B) Effects of SMI on the cytotoxicity of ADR to LoVo cells. (C) Effects of SMI on the cytotoxicity of PTX to Caco-2 cells. (D) Effects of SMI on the cytotoxicity of PTX to LoVo cells. *P<0.05 between 1 μL/mL of SMI co-treated group versus the group treated with chemotherapeutics alone. #P<0.05, ##P<0.01 between 2 μL/mL of the SMI co-treated group versus the group treated with chemotherapeutics alone. (E) The effects of SMI on cell apoptosis induced by ADR or PTX. Colon cancer cells were incubated with ADR (500 nmol/L) or PTX (300 nmol/L) combined with or without SMI (2 μL/mL) for 24 h. Apoptosis was assessed using Hoechst 33258, and apoptotic features were assessed by observing the chromatin condensation and fragments staining (×200). Scale bar=50 μm. (F, G) Detection of apoptotic and necrotic cells via flow cytometry. **P<0.01. Data are presented as the mean±SEM from five independent experiments.