Figure 3. The sequence alignment of flavivirus NS1 β-ladders.

An α-helix is marked with a cyan line, β-strands are marked with magenta lines. Disulfide bonds are connected with green lines. In the alignment, conserved residues are black; similar residues are blue; variable residues are purple. Black dots, the 10-digit residues; orange dots, residues at the dimer interface; blue dots, epitope residues recognized by 22NS1²⁴; black dash line, epitope for pathogenic antibodies⁴³; black triangles, the residues mutated in Natal_RGN strain⁶.