Figure 1. PAK4 is highly expressed in breast cancer cell lines.

PAK4 protein levels in seven breast cancer cell lines were assessed by western blot analysis. β-actin was used as a loading control. 3T3 PAK4 WT and 3T3 PAK4 KO are 3T3 cells isolated from wild-type and PAK4 knockout mice, respectively. Knockout cells are used here only for the accurate identification of the PAK4 band. (The membranes were cut prior to exposure so that only the portion of gel containing bands in the size range of PAK4 or β-actin would be visualized, as described in materials and methods).