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. 2017 Feb 15;7:42555. doi: 10.1038/srep42555

Figure 3. Treatment with KPT-8752 and KPT-9274 leads to a decrease in cell proliferation in several breast cancer cell lines.

Figure 3

Cells were plated overnight and treated with DMSO (control), KPT-8752 or KPT-9274 from Day 0 to Day 4. The MTT colorimetric assay was then conducted where the cells were incubated with MTT solutions at different time points. MTT absorbance curves were then analyzed in MDA-MB-231 (A) MDA-MB-468 (B) SUM159 (C) MCF7 (D) Sk-Br3 (E) BT-474 (F) WT iMMEC (control) (G) and NIH3T3 (control) cells (H). The results, presented as change in absorbance over time, correlate with the number of viable cells over time and can be considered as an indicator of cell proliferation. (Note, the amount of MTT-I absorbed by each cell type varies, and therefore the overall growth rates cannot be compared from one cell type to another). Error bars represent SEM. Data shown is representative of three separate repeat experiments.