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. 2017 Feb 15;7:42590. doi: 10.1038/srep42590

Figure 5. Uch37 is required for the expression of ventrolateral mesoderm genes by promoting zygotic Wnt signalling during Xenopus gastrulation.

Figure 5

(a) Expressions of ventrolateral mesoderm genes including MyoD, Xpo, and Vent1. Vegetal-view of stage 10.5 embryos, MOs and mRNAs were unilaterally injected at four-cell stage embryos. β-galactosidase (LacZ) mRNA was co-injected to trace the injected site (Inj., injected site). (20 ng Co MO; 20 ng Uch37 MO; 100 pg Lef1; 2 ng Re.Uch37; 300 pg β-galactosidase). See also Table S4. (b) Expressions of Vent1 and Xpo. Vegetal-view of stage 10.5 embryo, Four-cell stage embryos were injected in VMZ and cultured until stage 10.5. Left panel, Co MO (40 ng); middle and right panels, injection of pCSKA-Wnt8 plasmids (500 pg) along with either Co MO (40 ng) or Uch37 MO (40 ng). See also Table S5. (c) RT-PCR analysis using animal cap explants. Four-cell stage embryos were animally injected with indicated reagents (20 ng Co MO; 20 ng Uch37 MO; 300 pg pCSKA-Wnt8; 1 ng Uch37 mRNA (WT); 1 ng a catalytically inactive Uch37 mRNA (IN)). Animal cap explants were dissected at stage 9, and then cultured in activin (5 ng/ml)–treated 1xMR until stage 11. Gsc, a dorsal mesoderm marker; Xpo, Vent1, and Vent2, ventrolateral mesoderm markers. Full images are presented in Supplementary information (Fig. S12).