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. 2017 Feb 15;7:42504. doi: 10.1038/srep42504

Figure 3. JA induces SF3B1 and SF3B3 protein expression and induces mRNA splicing errors.

Figure 3

(A) MCF-7 and MDA-MB-468 cells were treated with 1 μM of JA for 6, 12, 24 and 48 h, followed by immunoblotting analyses. Increased accumulation of SF3B1 and SF3B3 was detected following treatment with JA. Cropped blot is shown from one representative experiment. Full-length gels are included in the Supplementary information file. (B) JA induced mRNA splicing errors in MCF-7 and MDA-MB-468 cells. Cells were treated with 1 μM of JA for 6, 12, 24 and 48 h. Unspliced pre-mRNA was quantified using qPCR targeting the intronic regions of RIOK3, CDKN1B, DNAJB1, and BRD2. Bars represent the means ± s.d. of at least 3 independent experiments. Asterisks (*) indicate statistical significance compared with vehicle cells (P < 0.01, Student’s t-test). Note the significant induction of protein and unspliced pre-mRNA in JA-treated cells as early as 6 h after treatment.