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. 2017 Feb 15;7:42491. doi: 10.1038/srep42491

Figure 6. Effect of PDI knockdown on spontaneous seizure activity in epileptic (6 week-post SE) rats.

Figure 6

(A) Representative EEGs for control siRNA- and PDI siRNA-infused epileptic animals. (B–D) Anticonvulsive effect of PDI siRNA on spontaneous seizure activity: (B) the mean seizure frequency, (C) seizure duration and (D) behavioral seizure score (Open circles indicate each individual value. Closed circles indicate mean value. *p < 0.05 vs. control siRNA; n = 5, respectively). PDI knockdown inhibits the spontaneous seizure activity in chronic epileptic rats. (E) Co-immunoprecipitation analyses of NR1 or NR2A interaction with PDI in control siRNA- and PDI siRNA-infused epileptic animals. M, molecular weight marker. (F) The quantitative analyses of co-immunoprecipitation of PDI bound to NR1 and NR2A (*p < 0.05 vs. control siRNA; n = 7, respectively). (G) Western blot data for the amounts of -SH + -SNO on NR1 and NR2A subunits in normal (N), control siRNA- and PDI siRNA-infused epileptic animals. M, molecular weight marker. (H,I) Quantification of effects of PDI siRNA on the amount of -SH + -SNO on NR1 and NR2A subunit and PDI expression in epileptic rats (mean ± S.E.M.; *p < 0.05 vs. normal; n = 7, respectively). PDI siRNA infusion reduces the binding of PDI to NMDAR, the amount of -SH + -SNO of NMDAR subunits and PDI expression level in epileptic animals.