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. 2017 Feb 8;8:14419. doi: 10.1038/ncomms14419

Figure 5. Diminished β5t expression in cTECs of site #13 mutant mice.

Figure 5

(a–c) Histograms show the expression of β5t (a), MHC class I (b) and MHC class II (c) in cTECs and mTECs of wild-type (black lines), heterozygous mutant (blue lines), homozygous mutant (red lines) and β5t-deficient (grey shades) mice at 2 weeks old. Bar graphs show the relative fluorescence intensity (RFI, n=4) of β5t (a), MHC class I (b), and MHC class II (c) expression normalized to the mean fluorescence intensity measured in wild-type cells. *P<0.05; ***P<0.001; NS, not significant. See also Supplementary Fig. 2b. (d) Relative mRNA levels (means±s.e.m., n=3) of β5t, Foxn1, MHC I and MHC II in cTECs isolated from 2-week-old mice were measured by quantitative reverse transcription–PCR. mRNA levels were normalized to those of Gapdh mRNA levels and are shown relative to the levels in wild-type cTECs. **P<0.01; ***P<0.001. All statistical analyses were performed by student's t-test.