Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Feb 8;8:14419. doi: 10.1038/ncomms14419

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

(a) Flow cytometric analysis of thymocytes from 2-week-old mice. Shown are dot plots for CD8 and CD4 expression (left) and TCRβ expression (middle) in PI^- viable cells and dot plots for CD8 and CD4 expression in PI^- TCRβ^high cells (right). Bar graphs show cell numbers (means±s.e.m., n=4–6) of indicated thymocyte populations. (b) Flow cytometric analysis of splenocytes from 2-week-old mice. Histograms show TCRβ expression in PI^- viable cells. Dot plots show CD8 and CD4 expression in PI^- TCRβ^high cells. Bar graphs show numbers (means±s.e.m., n=4–6) of CD4⁺CD8⁻TCRβ^high T cells and CD4⁻CD8⁺TCRβ^high T cells. Numbers in dot plots and histograms indicate frequency of cells within indicated area. *P<0.05; **P<0.01; ***P<0.001; NS, not significant. Statistical analyses were performed by student's t-test. See also Supplementary Fig. 2c,d.