Figure 2. Topical tacrolimus decreases inflammation after lymphatic injury.

(a–c) Representative × 40 images of tail tissue sections from control and topical tacrolimus-treated animals 6 weeks after surgery with immunofluorescent localization of CD45⁺(a), CD4⁺ cells (b) and IFN-γ⁺ cells (c). Lymphatic vessels are stained for LYVE-1⁺ (red) in each figure. Quantifications of positive cell per 0.25 mm² area (four random areas per mouse) for both early and late treatments are shown to the right of each figure (P<0.001 for all; n=8/group). Experiments were repeated two to three times. All data represent mean±s.d. with P≤0.05 considered as significant. Data analysed by two-tailed Student's t-test. Scale bars, 100 μm.