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. 2016 Dec 29;121(3):1507–1514. doi: 10.1021/acs.jpcc.6b11235

Figure 1.

Figure 1

Illustration of the fluorescence dynamics between OG and mC arising from the association of GSH and GST. Free and bound OG-GSH is excited by two-photon excitation at 880 nm. This causes intrinsic OG and sensitized (FRET excited) mC fluorescence which collectively span 490–750 nm. Time-resolved fluorescence intensity and anisotropy measurements are made in two spectral windows illustrated by the gray areas (filter transmission curves) superimposed on the OG (solid line) and mC (dotted line) emission spectra. Donor window measurements report solely OG fluorescence dynamics (spontaneous emission from free OG-GSH and spontaneous emission plus nonradiative FRET transfer from OG-GSH-GST-mC). The contribution from OG (donor) bleed through in the acceptor window leads to more complex intensity and anisotropy dynamics, necessitating the new approaches applied in this work.