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Donor window fluorescence intensity decay of a mixture of 60 μM OG-GSH and 30 μM GST-mC with 880 nm excitation. The decay departs from the single exponential found for OG-GSH and is best fit to a biexponential decay with the 1.33 ns lifetime corresponding to the OG population undergoing FRET with an interacting fraction FI = (F1I+F2I) = 0.136(±0.005). The lifetimes yield a FRET rate of 0.51(±0.04) ns–1.
