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. 2016 Apr 26;26(6):392–416. doi: 10.3109/15376516.2016.1170251

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© 2016 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial NoDerivatives License (http://creativecommons.org/Licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

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Figure 9. — Experimental design to test the nebulized aerosols generated from the various test mixtures. A, An illustration of the generation of aerosols from the various test items using the Collison nebulizer and of the exposure set-up using the Vitrocell exposure system. The 3D bronchial epithelial cultures (reconstituted from one donor) were exposed to three different aerosols generated by nebulizing PG.G, PG.G/Nic, or sbPBS mixtures. B, The experimental groups. C, Various endpoints measured within the third-layer assessment. Abbreviations: AK: adenylate kinase; Nic: nicotine; PBS: phosphate-buffered saline; PG.G: propylene glycol and glycerol; PG.G/Nic: propylene glycol, glycerol, and nicotine; sbPBS: smoke-bubbled PBS.