Figure 3. Subcellular localization of HpaM by western blot analysis.

Xcc strains were cultured to an OD₆₀₀ of 1.0 and proteins were prepared using the method described by Feilmeier and associates (2000) (A) or the method described by Chen and associates (2010) (B). 30 (for total protein) or 10 μg of protein sample was separated by SDS-PAGE electrophoresis and transferred to a PVDF membrane. The presence of HpaM was detected by anti-His₆ monoclonal antibody. The histidine sensor kinase HpaS and the transcription regulator HpaR1 were used as controls. HpaM, protein sample was prepared from strain ΔhpaM/pRhpaMH6; HpaS, protein sample was prepared from strain ∆hpaS/pRhpaSH6; HpaR1, protein sample was prepared from strain ∆hpaR1/pRhpaR1H6.