Figure 6. Evidence from western blot analysis reveals that HpaM, HrcC and HrcJ are outer and inner membrane-bound proteins, respectively.

The outer and inner membrane fraction proteins from strain ∆hrcC/pRhrcCH6 (for HrcC detection), ∆hrcJ/pRhrcJH6 (for HrcJ detection), and ΔHpaM-HrcC/pRhpaMH6 (for HpaM detection) were prepared. 10 μg of protein for each sample was separated by SDS-PAGE electrophoresis and transferred to a PVDF membrane. The presence of HrcC, HrcJ, and HpaM was detected by anti-His₆ monoclonal antibody. The histidine sensor kinase HpaS (from strain ∆hpaS/pRhpaSH6) was used as a control.