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. 2017 Feb 15;7:42903. doi: 10.1038/srep42903

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Copyright © 2017, The Author(s)

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Additional tests using (A) LAMP and (B) CPA tests using field samples from 17 ASF cases in wild boar and 3 outbreaks in pigs (lanes 1–20). 21 – blood collected from a non-infected pig, 22 – cDNA extracted from a classical swine fever virus (CSFV) strain Alfort/187, 23 – positive control standard ASFV plasmid containing a p72 gene fragment (7.2 × 10⁷ copies μl⁻¹); 24 – cDNA extracted from a porcine reproductive and respiratory syndrome virus (PRRS) strain Lelystad (genotype I). M – molecular length marker GeneRuler 100 bp DNA Ladder Plus (Thermo-scientific, Waltham, Massachusetts, USA).