FIG 1.

(A) Genomic positions of 4.5S RNA in M. tuberculosis (mtu) and C. glutamicum R (cgl). (B) Growth of the R strain (wild type) harboring pCRB52iP (vector plasmid), the ΔybeY strain harboring pCRB52iP, and the ΔybeY strain harboring pCRB52iP-ybeY. For the growth curve, overnight cultures of the wild type harboring pCRB52iP (circles), the ΔybeY strain harboring pCRB52iP (triangles), and the ΔybeY strain harboring pCRB52iP-ybeY (squares) grown on A-rich medium containing 1% glucose were washed and inoculated into fresh A-rich medium containing 1% glucose and 1 mM IPTG (isopropyl-β-d-thiogalactopyranoside). Cell growth was monitored by measuring the OD₆₀₀. Average values and standard deviations from at least three independent cultivations are shown.