FIG 3.

Mapping of the 5′ end of 4.5S RNA precursors. (A) Primer extension analysis of 4.5S RNA from wild-type and Δpnp, ΔrneG, and ΔybeY mutant cells. Primer extension products were derived from the total RNAs of wild-type and Δpnp, ΔrneG, ΔybeY, and ΔrneG Δpnp mutant cells grown on A-rich medium containing 1% glucose medium and separated on a denaturing polyacrylamide gel along with sequencing ladders (C, T, G, and A) obtained using the same primer. Parts of the sequence around the 4.5S RNA coding region are indicated on the left side. The transcriptional start site (TSS) is indicated by arrows. (B) Promoter sequence of 4.5S RNA. The genomic positions of 4.5S RNA and parts of the sequence around the promoter region of 4.5S RNA are shown. The transcriptional start site of the 4.5S RNA is defined as position +1. The experiment was carried out 4 times.