TABLE 1.
Position of 3′ end | No. of clones |
|||
---|---|---|---|---|
R | Δpnp mutant | ΔrneG mutant | ΔybeY mutant | |
+89 | 66 | 35 | 27 | 5 |
+90 | 0 | 0 | 0 | 8 |
+91 | 0 | 0 | 0 | 3 |
+92 | 0 | 0 | 0 | 7 |
+93 | 0 | 1 | 0 | 0 |
+94 | 0 | 0 | 1 | 0 |
+95 | 0 | 0 | 1 | 0 |
+96 | 1 | 0 | 0 | 0 |
+97 | 1 | 1 | 2 | 4 |
+98 | 0 | 1 | 1 | 2 |
+99 | 0 | 0 | 1 | 5 |
+100 | 11 | 17 | 2 | 32 |
+101 | 0 | 1 | 0 | 4 |
+102 | 2 | 2 | 1 | 8 |
+103 | 0 | 0 | 3 | 0 |
+123 | 0 | 0 | 6 | 0 |
+124 | 7 | 0 | 38 | 10 |
+125 | 0 | 0 | 7 | 0 |
+132 | 14 | 46 | 10 | 15 |
Total | 104 | 104 | 100 | 103 |
For each strain, at least 100 clones of each 3′ RACE-PCR product were sequenced. The numbers of sequenced clones are indicated in each column. The transcriptional start site of the 4.5S RNA transcript is defined as position +1.