TABLE 2.
Strains and plasmids used in this study
| Strain or plasmid | Relevant genotype or description | Reference or source |
|---|---|---|
| Strains | ||
| E. coli | ||
| HST02 | F′ [traD36 proA+B+ lacIq lacZΔM15] Δ(lac-proAB) recA endA gyrA96 thi Δe14 [Δ(ymfJ-mcrA)] supE44 relA ΔdeoR Δ(mrr-hsdRMS-mcrBC) | TaKaRa |
| SCS110 | rpsL (Strr) thr leu endA thi-1 lacY galK galT ara tonA tsx dam dcm supE44D (lac-proAB) [F′ traD36 proAB lacIq lacZΔM15] | TaKaRa |
| C. glutamicum | ||
| R | Wild-type strain JCM18229 | 31 |
| ΔrneG mutant | R ΔrneG | 22 |
| Δrnc mutant | R Δrnc | 21 |
| Δpnp mutant | R Δpnp | 21 |
| Δrnj mutant | R Δrnj | Takemoto et al., unpublisheda |
| ΔrnR mutant | R ΔrnR | This study |
| ΔrnZ mutant | R ΔrnZ | This study |
| ΔybeY mutant | R ΔybeY | This study |
| ΔrneG Δpnp mutant | R ΔrneG Δpnp | This study |
| ΔrneG ΔybeY mutant | R ΔrneG ΔybeY | This study |
| FFHT1 | R with FLAG-tagged ffh | This study |
| FFHT2 | Δpnp mutant with FLAG-tagged ffh | This study |
| FFHT3 | ΔrneG mutant with FLAG-tagged ffh | This study |
| FFHT4 | ΔybeY mutant with FLAG-tagged ffh | This study |
| FFHT5 | ΔrneG Δpnp mutant with FLAG-tagged ffh | This study |
| Plasmids | ||
| pCRA725 | Kmr; suicide vector containing the Bacillus subtilis sacB gene | 33 |
| LKSΔrnR | pCRA725 with pnp fragment | This study |
| LKSΔrnZ | pCRA725 with rnZ fragment | This study |
| LKSΔybeY | pCRA725 with ybeY fragment | This study |
| LKSffh-FLAG | pCRA725 with a gene encoding Ffh-FLAG | This study |
| pCRB52iP | E. coli-C. glutamicum shuttle vector; Kmr ColE1 ori pBY503 ori tac promoter lacIq | 21 |
| pCRB52iP-ybeY | pCRB52iP with ybeY | This study |
| pSPT18 | Apr; pUC18-based vector containing T7 and SP6 promoters | Roche |
| pGEM-T Easy | Apr; vector | Promega |
a
N. Takemoto, Y. Tanaka, T. Maeda, N. Hamamoto, and M. Inui, unpublished data.