FIG 10.

IL-2 administration following Ad5 immunization restores Th1 differentiation. (A) Experimental setup. SMARTA CD4 T cells were transferred into C57BL/6 mice that were subsequently immunized with Ad5 vectors. A dose of 15,000 IU of recombinant IL-2 or PBS was injected intraperitoneally (i.p.) into the mice every 12 h from the day of immunization and until day 7 postimmunization, before sacrifice at day 8. (B) Representative FACS plots showing the phenotype of SMARTA CD4 T cells in the spleen. (C and D) The frequency of Th1 (C) or Tfh (D) SMARTA CD4 T cells. Data are representative of 2 independent experiments with 3 to 4 mice per group per experiment. Error bars indicate standard errors of the means. ***, P < 0.001; ****, P < 0.0001.