FIG 3.
Immunization with Ad5 vectors does not generate Th2 or Th17 cells. SMARTA chimeric mice were generated and immunized with Ad5-LCMV GP vectors or infected with LCMV. At day 8 postimmunization or infection, SMARTA CD4 T cells were analyzed for Th2-associated (A) or Th17-associated (B) transcription factors and cytokines. (A) Representative FACS plots show GATA3 and IL-4 expression levels. (B) Representative FACS plots show RORγt and IL-17 expression levels. Th2 positive-control cells expressing GATA3 and IL-4 and Th17 positive-control cells expressing RORγt and IL-17 were analyzed together. Cytokine production was measured after ex vivo stimulation with GP61-80 peptide.