FIG 5.

Localization of AC83HA and AC83 deletion 1NΔ+CΔ by Western blotting in ODV and BV envelope and nucleocapsid fractions. (A, left) Detection of full-length AC83 in total ODV (ODV) and envelope (ENV) and nucleocapsid (NC) fractions using anti-HA antibody. The majority of AC83HA is located in the ODV envelope, but a reduced amount is always found in the nucleocapsid fraction. (Right) Comparison of the fractionation of AC83HA to that of 1NΔ+CΔ. Unlike AC83HA, 1NΔ+CΔ AC83 is only located in the nucleocapsid fraction. (B) Western blot analysis of AC83HA isolated from ODV in the presence (PI) and absence of protease inhibitors. The blot shows that some of the AC83HA in the nucleocapsid fraction is cleaved in the absence of protease inhibitors. (C) Western blot analysis of BV as well as the envelope and nucleocapsid fractions of BV. The quality of the fractionation shown in panels A, B, and C was confirmed by Western blotting of the same samples using antisera against the known nucleocapsid protein VP39 and the ODV envelope protein ODV-E25 or the BV envelope protein GP64 (shown at the bottom).