FIG 5.

Illustration showing the assembly mechanism of influenza virus RNA-dependent RNA polymerase (RdRp) using BiLC. Plasmids expressing PA-GC and PB2-GN (A), GN-PB1 and PA-GC (B), or PB1-GN and PB2-GC (C) were cotransfected with empty vector or with vector expressing Flag-PB1 (A), Flag-PB2 (B), or Flag-PA (C), respectively. The RLU values of cell lysates were determined by the use of a microplate reader at 24 h posttransfection. The levels of protein expression were detected by Western blotting as indicated. Data are shown as means ± SEM. **, P < 0.01; ***, P < 0.001 (Student's t test).