TABLE 2.

K_d, K_on, and K_off values of nsp10-nsp16 interaction measured by Octet biolayer interferometry in the presence or not of SAM or SAH^a

Condition(s)	Mean ± SD
	Kd, nsp10-nsp16 interaction (μM)	Kon (M−1 s−1)	Koff (s−1)
Without SAM, SAH	1.24 ± 0.015	3.77 × 103 ± 4.35	4.68 × 10−3± 0.016 × 10−3
With 100 μM SAM	0.15 ± 0.001	3.98 × 103 ± 2	6.23 × 10−4 ± 0.031 × 10−4
With 20 μM SAH	0.48 ± 0.003	3.10 × 103±1.97	1.51 × 10−3 ± 0.004 × 10−3
With 100 μM SAH	0.19 ± 0.0013	3.3 ×103± 2.2	6.51 × 10−4 ± 0.041 × 10−4

^aSee also Fig. 6A.