FIG 2.

Vif binding site of A3H consists of α-helixes α3 and α4. (A) A3H residues that conferred resistance to HIV Vif are highlighted in red on the A3H protein surface and on α-helixes α3 and α4. (B) Single-cycle infectivity assays with selected A3H alanine mutants. The average relative infectivity values for a triplicate transfection are shown. Error bars represent the standard deviations. (C) Summary of the A3H mutations that confer resistance to Vif. An asterisk indicates the A3H residue positions that do not disrupt its interaction with Vif when mutated to an alanine.