FIG 1.
Soluble CD4 (sCD4) and CD4mc inhibition of HIV-1YU2 Env variants with alterations of the gp41 ectodomain. Recombinant HIV-1 strains expressing luciferase and bearing wild-type or mutant HIV-1YU2 Envs were normalized by reverse transcriptase activity. Equal amounts of viruses were incubated with serial dilutions of sCD4 (A), JP-III-48 (B), or DMJ-II-121 (C) at 37°C for 1 h prior to infection of Cf2Th-CD4 CCR5 cells. Infectivity at each dilution of sCD4 or inhibitor tested is shown as the percentage of infection without sCD4 or inhibitor for each particular mutant. Quadruplicate samples were analyzed in each experiment. Data shown are the means of results obtained in at least 4 (A) or 3 (B and C) independent experiments. The error bars represent the standard deviations.