FIG 3.
Effect of gp41 ectodomain changes on inactivation mediated by ligands that prefer the unbound conformation of HIV-1 Env. Recombinant HIV-1 expressing luciferase and bearing wild-type or mutant Envs from HIV-1YU2 (A and B) or HIV-1HXBc2 (C and D) were normalized by reverse transcriptase activity. Equal amounts of viruses were incubated with serial dilutions of BMS806 (A and C) or 484 (C and D) at 37°C for 1 h prior to infection of Cf2Th-CD4 CCR5 (A and B) or Cf2Th-CD4 CXCR4 cells (C and D). Infectivity at each dilution of inhibitor tested is shown as the percentage of infection without inhibitor for each particular mutant. Quadruplicate samples were analyzed in each experiment. Data shown are the means of results from 4 (A), 3 (B), or 2 (C and D) independent experiments. The error bars represent the standard deviations.