FIG 2.

Analysis of HMGA1⁺ neurons that express β-catenin or ORF2 in consecutive sections. (A) Consecutive sections from TG of calves latently infected with BoHV-1 were prepared, and one section was stained with an antibody that recognizes HMGA1. The adjacent section was stained with an antibody that recognizes β-catenin. The neurons numbered 1 to 3 were stained with both antibodies in adjacent sections. The letters a to d denote neurons that were stained by the HMGA1 antibody but not the β-catenin antibody. These results are representative of those for TG from 2 different calves latently infected with BoHV-1. (B) Dually stained TG neurons in adjacent sections. The number of β-catenin⁺ or ORF2⁺ neurons among 150 HMGA1⁺ neurons was calculated, and the results are expressed as the percentage of neurons stained by both antibodies. (C) TG sections were counterstained with methyl green. The percentage of neurons in which the nucleus was clearly detected was counted. For this study, 400 TG neurons from a calf latently infected with BoHV-1 and TG from an uninfected calf were counted.