FIG 1.

Effect of extending neutralization times on reversibility of low-pH inactivation of HSV-1 infectivity. Extracellular HSV-1 KOS virions were treated with pH 7.4 or 5.0 for 20 min at 37°C. Samples were neutralized to pH 7.4 for the time indicated, and titers were determined on Vero cells. At 18 to 24 h postinfection, titers were determined by plaque assay. Infectivity of pH 7.4-treated virions was set to 100%. Data are the means from four replicates with standard errors. The P values were determined using Student's t test (*, P < 0.002). Results are representative of three independent experiments.