FIG 6.

IRAV relocalizes to the replication complex after DENV infection. (A) Confocal microscopy of XRN1 colocalized with IRAV and DENV NS3 at the replication complex in DENV-infected or mock-infected A549 cells. Green, IRAV; magenta, XRN1; red, DENV NS3. The nucleus was stained with DAPI (blue). Colocalization between IRAV, XRN1, and DENV NS3 is shown in white. Regions of interest (ROI) are boxed in white. (B) Colocalization coefficients of IRAV and DENV NS3 or IRAV and XRN1 in DENV-infected A549 cells as determined by Pearson's linear correlation coefficient, demonstrating colocalization between IRAV and both XRN1 and DENV NS3 in DENV-infected cells. The error bars represent standard deviations.